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. 2019 Feb 25;9:2678. doi: 10.1038/s41598-019-39591-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Scatter plot of log2-transformed protein fold change (SILAC, n = 1, 2702 unique protein hits passing QC) vs cytoplasmic transcript log2-transformed fold change (RNA-Seq - DESeq. 2, n = 2). Pearson correlation = +0.294. Red = FC > 2 | Blue = FC < −2 in both comparisons. (B) Venn diagram representing overlap in genes identified as being depleted in the cytoplasm (FDR < 0.05) and down at the protein level by SILAC, comparing SF3B1^K700E vs wildtype samples. (C) Network of directly interacting proteins based on those identified as being decreased in the SF3B1^K700E cells by SILAC. Nodes coloured according to cluster assigned by Reactome FI. PPIs identified by STRING-DB (direct, 0.5 confidence). Table identifies top 4 significantly enriched gene ontology terms from Reactome, all related to translation (green cluster). (D) Network of directly interacting proteins based on those identified as being increased in the K700E cells by SILAC. Nodes coloured according to cluster assigned by Reactome FI. PPIs identified by STRING-DB. Table identifies top 4 significantly enriched gene ontology terms from Reactome, including oxygen transport (red) and splicing (purple). (E) Flow cytometry histograms of cellular fluorescence representing OP-Puromycin (OP-Puro) uptake in wildtype, +/Δ/Δ and +/+/K700E cells.