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. 2019 Feb 25;9:2657. doi: 10.1038/s41598-019-39162-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TMVcr and TMVcr-Δ122 retain infectivity in tobacco, after passaging in mycelia of P. infestans. (a) Mosaic and severe leaf deformation and mild mosaic (b) shown by tobacco plants rub-inoculated with plant sap obtained from leaves of plants infected by TMVcr and TMVcr-Δ122, respectively, compared with those induced by rub-inoculation with mycelia collected from liquid cultures of P. infestans at 10 dpi with TMVcr (c) and TMVcr-Δ122 (d). (e) Mock-inoculated tobacco plants.