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. 2019 Feb 25;9:2657. doi: 10.1038/s41598-019-39162-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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P. infestans produces virus-specific siRNAs in response to viral infection. Detection of virus-specific siRNAs in total RNA preparations enriched in sRNAs extracted from mycelia of P. infestans at 10 dpi with specific for TMVcr (cr) and TMVcr-Δ122 (Δ122). These bands were not detected in RNA preparations extracted from mycelia of P. infestans not exposed to co-incubation with viral preparations (WT). Panel (a) shows an image of the Gel red-stained 15% polyacrilamide gel as loading control. Panel (b) shows hybridization of hydrolyzed DIG-RNA probes for TMVcr replicase and CP. Arrowheads point migration of 21-nt ssDNA primers as size markers (panel a) and migration of siRNAs (panel b).