Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 25;10:925. doi: 10.1038/s41467-019-08609-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Crown 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Structure of human TTR and experimental studies of TTR stability. a–c Crystallographic structure of WT-TTR (PDB-ID 4PVM). Tafamidis is shown in green (by superimposition of PDB-ID 6FFT), the S52 amino acid in red, and T119 in blue. d Scheme of the native MS experiment: 4H (hydrogenated) and 4D (deuterated) tetramers are mixed in equal parts; following dissociation, four hybrid tetrameric species may form by the assembly of monomeric and dimeric species. e–h MS result for the subunit exchange between deuterated S52P and hydrogenated WT, S52P, T119M, and S52P bound to tafamidis, respectively. Changes in relative abundance of homo- and hetero-tetrameric species over the course of 11 days are shown, along with an estimate of the of their association/dissociation rates (uncertainties are one standard deviation of the posterior densities)