FIGURE 5.

Immunophenotyping of T cells in splenocytes of F1 and F1-CD44^ASK mice. In the F1 mice, consequence of the allele specific knockout of CD44 was analyzed by assessing the alteration of phenotype which was observed in PWD parental strain. (A) The gating method for T cells in the spleen of F1 and F1-CD44^ASK mice. (B) Frequency of CD44^lowCD62L^high cells (R1) and CD44^highCD62L^high cells (R2) in CD4⁺CD8^- cells of F1 and F1-CD44^ASK mice. (C) Frequency of CD44^lowCD62L^high cells (R3) and CD44^highCD62L^high cells (R4) in CD4^-CD8⁺ cells of F1 and F1-CD44^ASK mice. (D) MFI of CD44 positive cells in CD4⁺ cells of F1 and F1-CD44^ASK mice. (E) MFI of CD44 positive cells in CD8⁺ cells of F1 and F1-CD44^ASK mice. Representative FACS data were from two independent experiments involving at least six mice for each genotype. Data were analyzed by two-tailed Student’s t-test. Data were presented as Mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.