Figure 3.

Effect of melatonin on IgG level. (a) Effect of melatonin on the expression of IgG in LPS-stimulated mouse mammary tissue. IgG protein was analyzed by Western blotting. (b) Relative abundance of IgG protein in LPS-stimulated mouse mammary tissue. Proteins were normalized with β-actin. (c) Effect of melatonin on the production of IgG in tissue culture medium. IgG protein was analyzed by Western blotting. (d) Relative abundance of IgG protein in tissue culture medium. Control: containing ethanol as the solvent at the same concentration used in the tests. LPS: a working concentration 100 ng/mL LPS. Mel: a working concentration 10 μg/mL melatonin. LPS+Mel: final concentrations of 100 ng/mL LPS and 10 μg/mL melatonin. Values with different letters differ significantly (P < 0.05).