Fig. 3.

Sex-dependent FAs and PL profile in liver. Sex-dependent fatty acids (FAs) and phospholipid (PL) profile in liver of ob/ob F (♀ open bars and pink bullets) and M (♂ gray bars and blue triangles) mice. a Relative quantification of the most abundant FAs (n = 3–4) obtained by lipidomic analysis; b hepatic expression levels of elongases and desaturases genes; rRelative hepatic (c) phosphatidylcholine (PC), d lysophosphatidylcholine (LPC), e phosphatidylethanolamine (PE) and f lysophospatidylethanolamine (LPE) species content (n = 3–4); and g relative mRNA expression level of the PL synthesis pathway genes in F and M mice (n = 7–8). Values are means ± sem; *, P < 0.05, **, P < 0.01, ***, P < 0.001 and ****, P < 0.0001 M vs F were considered significant. Elovl fatty acid elongase, Scd fatty acid desaturase, Ck choline kinase, Ct choline transferase, Ek ethanolamine kinase, Et ethanolamine transferase, Pemt phosphatidylethanolamine N-methyltransferase and Lp-Pla2 Lipoprotein associated phospholipase A2