Fig. 2.

Analysis of the forkhead transcription factor, O subgroup, member 1 (FOXO1) gene mutation and transcript levels. None of the tumors in the Cancer Genome Atlas (TCGA) data set has a mutation in FOXO1 gene (a; n = 168). FOXO1 mRNA levels in pancreatic adenocarcinoma (PAC) tissue samples (b; n = 168) or in pancreatic carcinoma cell lines (c; n = 43). The data were normalized to mRNA levels in tumor samples or cell lines with wild-type KRAS, TP53, SMAD4 and CDKN2A. Data are presented as means and error bars represent the SD of the mean. d Kaplan scan for FOXO1 derived from clinically annotated human pancreatic cancer gene expression set (Yey: 4hm44K, http://hgserver1.amc.nl/cgi-bin/r2/main.cgi). Patients with low FOXO1 expression in their tumor tissue had significantly shorter overall survival after 48 months than patients with high FOXO1 levels (p = 0.048, log-rank test).