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. 2019 Feb 11;15(2):e1007574. doi: 10.1371/journal.ppat.1007574

Fig 3. The aggresome is important in TMEV replication.

Fig 3

(A, B) DA and GDVII virus-infected BHK-21 cells at 8 HPI. The aggresome in TMEV-infected cells contains VP1, ds-RNA (A) and L (B). L* is expressed in cytoplasm but outside of aggresome (B). (C) ds-RNA is present in small aggregates throughout the cytoplasm of DAΔL-infected BHK-21 cells at 8 HPI. (D) Nocodazole, a microtubule inhibitor, hampers aggresome formation in DA-infected BHK-21 cells at 8 HPI. (E) The amount of DA virus genome is decreased in a dose-dependent manner 6 HPI with two different MOIs in BHK-21 cells that had been treated with nocodazole (0, 3, 10, 30 μM) for 1h prior to infection. (F) Plaque assay of DA-infected BHK-21 cells treated with nocodazole treatment (10 μM, for 1h prior to infection) shows a decrease in virus titer compared to untreated cells. Scale bars: 10 μm.