(A, B) DA and GDVII virus-infected BHK-21 cells at 8 HPI. The aggresome
in TMEV-infected cells contains VP1, ds-RNA (A) and L (B). L* is
expressed in cytoplasm but outside of aggresome (B). (C) ds-RNA is
present in small aggregates throughout the cytoplasm of DAΔL-infected
BHK-21 cells at 8 HPI. (D) Nocodazole, a microtubule inhibitor, hampers
aggresome formation in DA-infected BHK-21 cells at 8 HPI. (E) The amount
of DA virus genome is decreased in a dose-dependent manner 6 HPI with
two different MOIs in BHK-21 cells that had been treated with nocodazole
(0, 3, 10, 30 μM) for 1h prior to infection. (F) Plaque assay of
DA-infected BHK-21 cells treated with nocodazole treatment (10 μM, for
1h prior to infection) shows a decrease in virus titer compared to
untreated cells. Scale bars: 10 μm.