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. 2019 Feb 26;17:17. doi: 10.1186/s12964-019-0327-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — CDK4 inhibition by palbociclib induced cell cycle arrest and apoptosis in chondrosarcoma cell lines. Human chondrosarcoma cells CS-1 and SW1353 were treated with increasing concentrations of palbociclib for the indicated time. After exposure to palbociclib (1 μM) for 24 h, the cell cycle was assessed by flow cytometry analysis. Representative images of cell cycle distribution in CS-1 (a) and SW1353 (b) with or without palbociclib treatment. (c and d) Different cell cycle phase rates were analyzed. (e) Flow cytometric analysis of palbociclib-treated CS-1 and SW1353 cells. (f) Percentage of apoptotic CS-1 and SW1353 cells