Fig. 5.

CDK4 inhibition induced by palbociclib suppresses cell migration and invasion. After exposure to 1 μM of palbociclib for the indicated time, the cell migration of CS-1 and SW1353 cells was determined by wound healing assay. (a) Representative images of CS-1 and SW1353 cell migration after palbociclib treatment for 0, 16, and 32 h (scale bar =50 μm). (b) Cell migration distance of CS-1 cells was measured after palbociclib treatment. *P < 0.05 compared with 0 h group. (c) Human chondrosarcoma cells CS-1 and SW1353 were starved for 12 h, and then seeded in the top chambers of transwells with matrigel in the presence of the indicated doses of palbociclib. The bottom chambers of the transwells were filled with a medium containing 10% FBS. Cancer cells were allowed to invade for 10–12 h. The invading purple-stained cells showing irregular shape were photographed and counted (scale bar =50 μm). (D) Quantitative analysis of the percentage of cell invasion using ImageJ. Columns represent the means of experiments performed in triplicate, where the bars represent the SD. **P < 0.01 compared with cell only group