Table 1.
Primer sequences for quantitative real-time polymerase chain reaction
| Gene | Forward primer | Reverse primer |
|---|---|---|
| IHH | CCAACTACAATCCCGACATCATCT | CTCGTCCCAGCCTTCAGTCA |
| SHH | CTGACCGTGACCGTAGCAAGT | TGGATGTGGGCTTTGGACTCA |
| Sox9 | CTGACCGTGACCGTAGCAAGT | TGGATGTGGGCTTTGGACTCA |
| ANCN | ATGGCTTCCACCAGTGCG | CGGATGCCGTAGGTTCTCA |
| collagen II | GCTCCCAGAACATCACCTACCA | ATTCCTGCTCAGGCCCTCC |
| collagen X | CCCTTCTGCTGCTAGTGTC | GTCTTGGTGTTGGGTTGT |
| Runx2 | CCTTCCACTCTCAGTAAGAAGA | TAAGTAAAGGTGGCTGGATAGT |
| annexin V | GCAGAACTAACAGCCATAA | AGAACCACCAACATCCTC |
| Beta-2-microglobulin | AACGTGGAACAGTCAGACC | AGTAATCTCGATCCCATTTC |