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. 2019 Feb 26;7:27. doi: 10.1186/s40478-019-0677-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Double immunofluorescent staining reveals FMRpolyG positive inclusion bodies in both astrocytes (a) and neurons (b) from Gfa2-CGG99 mice. a. Photomicrograph showing FMRpolyG-positive inclusion bodies (red) located within GFAP positive astrocytes (green; arrow) as well as in a GFAP negative cell that is probably neuronal (arrow head). b. Photomicrograph showing FMRpolyG-positive inclusion bodies (red) located within a MAP 2 positive (green; arrow) neuron as well as in a MAP 2 negative cell that is probably an astrocyte (arrow head). c Representative brain section from a Gfa2-CGG99 mouse processed for immunofluorescence but without 8FM mouse anti-FMRpolyG primary antibody