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. 2019 Feb 26;7:27. doi: 10.1186/s40478-019-0677-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Ectopic and intracytoplasmic ubiquitin-positive inclusions in Gfa2-CGG99-eGFP expressing astroglia. a Ubiquitin-positive astrocyte intranuclear inclusion (small arrow) and nearby inclusions that appear to be extracellular (arrowheads) in a neutral-red stained section of hilus from a Gfa2-CGG99 mouse. Insert a: Intranuclear (small arrow) and extracellular (arrowhead) ubiquitin-positive inclusions. DAB immuno-peroxidase staining was used to label ubiquitin-positive inclusions in A and inset a. b Intracytoplasmic ubiquitin-positive inclusion (arrowhead) in an eGFP histofluorescent astrocyte in the stratum oriens of hippocampal CA1 region; pcl pyramidal cell layer. Inset b shows higher magnification of the inclusion body (arrowhead). c An eGFP histofluorescent astrocyte from a Gfa2-CGG99 mouse with an intracytoplasmic ubiquitin-positive inclusion body (arrowhead) within the hippocampal CA1 stratum radiatum. d Ubiquitin-positive intracytoplasmic inclusion (arrowhead, yellowish-green fluorescence) that co-localizes with eGFP in the CA1 pyramidal cell layer (d). Inclusions were immunolabeled eGFP (red) and ubiquitin (green), so that co-localization with the inclusions appears as yellowish-green fluorescence. Note that eGFP histofluorescence from expression of the eGFP reporter gene (green) is also present. Nuclei were labeled with DAPI. d-f Co-localization of intracytoplasmic inclusions (arrowheads) in astrocytes immunofluorescently labeled with ubiquitin (green) and GFAP (red). eGFP histofluorescence from expression of the eGFP reporter gene (green) is also present. Nuclei were labeled with DAPI. Scale bars: a-f: 10 μm