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. 2019 Feb 26;6:190028. doi: 10.1038/sdata.2019.28

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Copyright © 2019, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.

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(a,b) Principle of the tetracycline-dependent regulatory (tTA/Tet-Off) system. A tissue-specific promoter drives spatially restricted expression of the tetracycline transactivator protein (tTA) in the desired tissue. In the absence of tetracycline (Tet), the tTA protein binds to a tetracycline responsive element (TRE) which in turn drives expression of the transgene of interest. (b) If tetracycline (Tet) is administered, it prevents tTA from binding to the TRE and activating transgene expression. (c) Outline of the workflow used for collection, analysis and presentation of experimental data.