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. 2019 Feb 26;6:190028. doi: 10.1038/sdata.2019.28

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Copyright © 2019, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.

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(a–d) Section images showing X-gal labeling in four Pitx3-tTA cases, two sectioned coronally (a,b) and two sectioned horizontally (c,d), with atlas overlay shown on one side. The positions of labeled cells were manually recorded from all section images by placing point coordinates on each labeled cell using the annotation tool in the LocaliZoom viewer (exemplified in the enlarged inset in a). (e–h) The point coordinates were color-coded and co-visualized together with atlas structures in a 3-D viewer. Views from anterolateral (e), dorsal (f), lateral (g), and anterior (h) are provided. In (e), the outer boundaries of the substantia nigra and ventral tegmental area are shown as transparent pink surfaces. The 3-D visualization allows comparison of data derived from coronal and horizontal section images, and demonstrates that labeled cells are similarly distributed in the four Pitx3-tTA cases, with high densities in the substantia nigra and ventral tegmental area, but also with labeling in more dorsal parts of the mesencephalon. Scale bar, 1 mm.