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. 2019 Mar;25(3):559–563. doi: 10.3201/eid2503.181282

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Neighbor Net splitstree of Mycobacterium chimaera isolates: relationships between M. chimaera isolates (n = 124) mapped against the M. chimaera strain CDC 2015–22–71 heater–cooler unit (HCU) reference genome (18,190 single nucleotide polymorphisms [SNPs] in 3,815,639 core positions). Isolates were grouped with a threshold of <500 SNPs to the nearest cluster. Clustered HCU isolates, including the reference strain CDC 2015-22-71, comprise the HCU1 cluster (n = 112) and HCU2 (n = 3). Unclustered isolates include Australian (AUS) HCU isolates (n = 2), USA non-HCU isolates (n=8), and the type strain FI-01069. Scale bar indicates SNPs.