Table 3.
Proteins identified by mass spectrometry
| Spot ID | Observed | Fold difference | Protein | Theoretical | Masscot Score | Coverage (%) | Confirmed Sequences (Masscot ion score) | Search log | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MW | pI | wRA | OA | HL | MW | pI | |||||||
| aRA | iRA | ||||||||||||
| 208 | 90 | 5.3 | 1.4* | 1.0 | 1.0 | Pro-neuregulin-3, membrane-bound isoform | 78 | 7.8 | 61 | 12 | 388QAKQIQEQLK397(19) | 38,220 | |
| NRG3_HUMAN | |||||||||||||
| 236 | 87 | 5.1 | 1.1 | 1.3* | 1.0 | Cathepsin F | 53 | 8.5 | 26 | 3 | 103TLLCSFQVLDELGRHVLLR121(26) | 38,210 | |
| CATF_HUMAN | peptide summary | ||||||||||||
| 325 | 65 | 5.1 | 0.9 | 0.7* | 1.0 | Ig alpha-2 chain C region | 37 | 5.7 | 132 | 10 | 251WLQGSQELPR260(53) | 38,207 | |
| IGHA2_HUMAN | 270QEPSQGTTTFAVTSILR286(70) | ||||||||||||
| 370 | 53 | 5.1 | 1.3* | 0.9 | 0.9 | 1.0 | Alpha-1-antitrypsin | 47 | 5.4 | 170 | 33 | 50ITPNLAEFAFSLYR63(79) | 38,190 |
| A1AT_HUMAN | 216GKWERPFEVK225(10) | ||||||||||||
| 494 | 40 | 7.0 | 0.7* | 0.9 | 0.9 | 1.0 | Keratin, type II cytoskeletal 1 | 66 | 8.2 | 62 | 9 | 212WELLQQVDTSTR223(14) | 38,206 |
| K2C1_HUMAN | 224THNLEPYFESFINNLR239(41) | ||||||||||||
| 564 | 24 | 5.2 | 6.3* | 1.2 | 1.1 | 1.0 | Toll-like receptor 3 | 104 | 6.7 | 68 | 19 | 532LEILDLQHNNLAR544(7) | 38,215 |
| TLR3_HUMAN | |||||||||||||
MW molecular weight, wRA whole RA, aRA active RA, iRA inactive RA, OA osteoarthritis, HL healthy donor
Fold differences are shown with the intensity of the protein spots in HLs defined as 1.0. Asterisks indicate that the spot intensity is significantly different (p < 0.05) from that in HLs