Fig. 4. The binding curves of compounds 2c and CPA (reference compound) are examples of A1 AR antagonistic action (A) and A1 AR agonistic action (B), respectively, determined via a GTP shift assays (with and without 100 μM GTP) in rat whole brain membranes expressing A1 ARs with [3H]DPCPX as radioligand. (A) GTP shift of 1.46 calculated for compound 2c, (B) GTP shift of 15 calculated for compound CPA.
