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27-OHC didn’t lead to ER alpha translocation to the nucleus. Treatment with estradiol (E2) leads to ERα translocation into the nucleus of Caco2 (A, white arrows) but not SW620 (B) cells. Treatment with either vehicle or 27-OHC didn’t lead to nuclear localization of ERα as evidenced by the presence of immunoreactivity to ERα antibody (red) in the cytoplasm but not in the nucleus (counter stained with DAPI (blue) in Caco2 (A) and SW620 (B) cells. Bar=10μm.
