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. 2019 Feb 22;14:1451–1467. doi: 10.2147/IJN.S193544

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© 2019 Xin et al. This work is published and licensed by Dove Medical Press Limited

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Bioactive release of irisin from i/SCS/P scaffold.

Notes: (A) Release kinetics of irisin from i/SCS/P. Effect of different concentrations of irisin on BMSCs proliferation was detected by CCK-8 and analyzed in OD values (B) and fold changes (C) compared to the corresponding control group. At each scheduled time, BMSCs cultured in standard medium without irisin were taken as control. (D) ALP activity assay. The i/SCS/P scaffold was immersed in culture medium for 4 hours, 12 hours, and 7 days, respectively. The extracts at different times were used to culture BMSCs for 3 days and then treated with ALP activity assay. Culture medium with a same concentration of irisin, corresponding to what was calculated in the extract, was used as positive control. *P<0.05, compared with the corresponding positive control group. **P<0.05, compared with the 4 hours and 12 hours collected medium. (E) ALP staining at 7 days and (F) ARS staining at 28 days. BMSCs were cultured with the different extracts of i/SCS/P. BMSCs cultured with the extracts of SCS/P scaffolds were used as control. Lower magnification images (scale bar: 1 mm) are shown in the upper panel. The region of interest is selected by a red or yellow box and magnified with higher magnification (scale bar: 500 µm) in the lower panel.

Abbreviations: CON, control; I, irisin; i/SCS/P, irisin-loaded silk/calcium silicate/sodium alginate/PVA; BMSCs, bone marrow stem cells; CCK-8, cell counting kit-8; ALP, alkaline phosphatase; ARS, alizarin red S; SCS/P, silk/calcium silicate/sodium alginate/PVA.

Bioactive release of irisin from i/SCS/P scaffold.

Notes: (A) Release kinetics of irisin from i/SCS/P. Effect of different concentrations of irisin on BMSCs proliferation was detected by CCK-8 and analyzed in OD values (B) and fold changes (C) compared to the corresponding control group. At each scheduled time, BMSCs cultured in standard medium without irisin were taken as control. (D) ALP activity assay. The i/SCS/P scaffold was immersed in culture medium for 4 hours, 12 hours, and 7 days, respectively. The extracts at different times were used to culture BMSCs for 3 days and then treated with ALP activity assay. Culture medium with a same concentration of irisin, corresponding to what was calculated in the extract, was used as positive control. *P<0.05, compared with the corresponding positive control group. **P<0.05, compared with the 4 hours and 12 hours collected medium. (E) ALP staining at 7 days and (F) ARS staining at 28 days. BMSCs were cultured with the different extracts of i/SCS/P. BMSCs cultured with the extracts of SCS/P scaffolds were used as control. Lower magnification images (scale bar: 1 mm) are shown in the upper panel. The region of interest is selected by a red or yellow box and magnified with higher magnification (scale bar: 500 µm) in the lower panel.

Abbreviations: CON, control; I, irisin; i/SCS/P, irisin-loaded silk/calcium silicate/sodium alginate/PVA; BMSCs, bone marrow stem cells; CCK-8, cell counting kit-8; ALP, alkaline phosphatase; ARS, alizarin red S; SCS/P, silk/calcium silicate/sodium alginate/PVA.