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. Author manuscript; available in PMC: 2019 Aug 6.
Published in final edited form as: Nature. 2019 Feb 6;566(7744):403–406. doi: 10.1038/s41586-019-0904-1

Extended Data Figure 8. Separation and detection of sapienate and cis-8-octadecenoate.

Extended Data Figure 8

(a) Separation and detection of sapienate (cis-6-hexadecenoate) and palmitoleate (cis-9-hexadecenoate) via gas chromatography mass spectrometry. Separation was optimized using a standard mix containing pentadecanoate, sapienate, palmitoleate, palmitate, cis-8-octadecenoate, oleate, vaccinate, linoleate and stearate (upper panel), and the method was subsequently validated by measurement of these fatty acids in biological samples. A representative biological sample is presented in the lower panel.

(b) Separation and detection of cis-8-octadecenoate, oleate (cis-9-octadecenoate) and vaccinate (cis-11-octadecenoate) via gas chromatography flame ionization detector. Separation was optimized using a standard mix containing cis-8-, cis-9- and cis-11-octadecenoate (upper panel), and the method was subsequently validated by measurement of these fatty acids in biological samples. A representative biological sample is presented in the lower panel.