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. Author manuscript; available in PMC: 2019 Aug 6.
Published in final edited form as: Nature. 2019 Feb 6;566(7744):403–406. doi: 10.1038/s41586-019-0904-1

Figure 3. Sapienate and its elongation product cis-8-octadecenoate are used in membrane synthesis.

Figure 3

(a,b) Relative fatty acid abundances in HUH7 and A549 cells treated 2 nM Merck Frosst Cpd 3j (n=3). Data were normalized to the respective controls.

(c) Palmitoleate and sapienate abundances in membrane phospholipids in HUH7 cells with non-targeting shRNA and an shRNA targeting FADS2 (n=4).

(d,e) Relative phospholipid-bound palmitoleate, sapienate and cis-8-octadecenoate abundances in HUH7 and A549 cells treated with control or 2 nM Merck Frosst Cpd 3j normalized to control (n=2 in e sapienate+SCD inhibitor; n=3 in all other cases,). BDL denotes ‘below detection limit’. When the respective control was BDL, the data were normalized to the total abundance of all fatty acids measured and are represented in arbitrary units.

Experiments were performed in low FBS (1% : HUH7; 0.5% : others) with treatment of 72 h. Error bars represent SD from mean of biological independent samples. Unpaired two-sided Student’s T-tests.