Table 3.

In silico site-directed mutagenesis and docking simulations for Ecdysone receptor. ^{a, b}

Mutant	Binding b	Predicted Mechanism	Same Side Chain Class	Molecular Weight Difference (g/mol)	Susceptibility Prediction
D506E	No change	None	Yes	14	Yes
D506P	Decrease	Electrostatic	No	18	Yes
T537A	No change	None	No	30	Yes
A592G	No change	None	Yes	14	Yes
A592F	Decrease	Steric hindrance	No	76	No
A592V	No change	None	Yes	28	Yes
N695A	Decrease	Steric hindrance	No	43	No

^aRaw data results are presented (Supplementary Data File).

^bAmino acids represented by acronyms is listed (Table 1).

^cBinding free energy of mutant model relative to binding free energy of wild-type model.