Skip to main content
. 2019 Feb 26;14(2):e0212063. doi: 10.1371/journal.pone.0212063

Fig 6. Dual-luciferase assay for miR-223-3P targeting STAT3 and for IL-6;miR-2909 targeting KLF4.

Fig 6

(A) predicted binding sites for miR-223-3P and STAT3, (B) changes in luciferase activity of added STAT3 3'-UTR pMIR-TM luciferase vector and miR-223-3p mimic, (C) STAT3 molecular level of added miR-223-3p mimic, (D) STAT3 protein level of added miR-223-3p mimic, (E) miR-223-3P and IL-6 predicted binding site, (F) changes in luciferase activity of added IL- 6 3'-UTR psi-CHECK2 luciferase vector and miR-223-3p mimic, (G) IL-6 molecular level of added miR-223-3p mimic, (H) IL-6 protein level of added miR-223 -3p mimic, (I) miR-2909 and KLF4 predicted binding sites, (J) changes in luciferase activity of added KLF4 3'-UTR pMIR GFP luciferase vector and miR-2909 mimic, (K) the molecular level of added KLF4 and miR-2909, (L) the protein level of added KLF4 and miR-2909. *** p <0.001; ** p <0.01; * p <0.05.