| LPS |
L-2880 |
Sigma |
| Standard fetal bovine serum |
SH30088.03 |
GIBCO |
| Dulbecco’s modified Eagle’s medium F12 |
SH30023.01 |
GIBCO |
| CCK8 kit |
PH0534 |
China Biyuntian Biotechnology Co., Ltd |
| BCA protein concentration assay kit |
NCI-3225 |
China Biyuntian Biotechnology Co., Ltd |
| site-directed mutagenesis kit |
KM101 |
China Biyuntian Biotechnology Co., Ltd |
| SDS-PAGE Sample Loading Buffer |
P0015 |
China Biyuntian Biotechnology Co., Ltd |
| horseradish peroxidase-labeled goat anti-rabbit antibody |
ORB175845, |
China Biyuntian Biotechnology Co., Ltd |
| Rabbit polyclonal anti-TLR2 antibody |
ab191458 |
Abcam |
| rabbit polyclonal anti-TLR4 antibody |
ab13556 |
Abcam |
| rabbit monoclonal anti-STAT3 antibody |
ab32500 |
Abcam |
| rabbit antibody anti-p-STAT3 |
Tyr-705 |
Abcam |
| rabbit monoclonal anti-NF-kB antibody |
ab59238 |
Abcam |
| rabbit monoclonal anti- p-NF-kB antibody |
ab16502 |
Abcam |
| rabbit monoclonal anti-KLF4 antibody |
ab80230 |
Abcam |
| mouse monoclonal anti-b-actin antibody |
HC201-01 |
Abcam |
| IL-6 Enzyme-linked immunosorbent assay (ELISA) kits |
E-EL-H0102 |
Wuhan Elitet Biotechnology Co., Ltd |
| IL-1β Enzyme-linked immunosorbent assay (ELISA) kits |
E-EL-H0149 |
Wuhan Elitet Biotechnology Co., Ltd |
| TNF-α Enzyme-linked immunosorbent assay (ELISA) kits |
E-EL-H0109 |
Wuhan Elitet Biotechnology Co., Ltd |
| human IL-6–stimulating factor |
PeproTech 96-200-06-5 |
Wuhan Elitet Biotechnology Co., Ltd |
| human TNF-a–stimulating factor |
PeproTech 96-300-01A-10 |
Wuhan Elitet Biotechnology Co., Ltd |
| p-STAT3 inhibitor |
S1155 S3I-201 |
Wuhan Elitet Biotechnology Co., Ltd |
| NF-kB inhibitor |
BAY 11–7082 |
Wuhan Elitet Biotechnology Co., Ltd |
| Real-time polymerase chain reaction (PCR) special reagent |
DRR802A |
TAKARA |
| SYBR Premix Ex TaqTM II (Perfect Real-Time) |
DRR081A |
TAKARA |
| Trizol |
10296010 |
Invitrogen |
| P004ECL luminescence detection kit |
W028-1 |
Vigorous |
| miR-223-3p mimics |
|
RuiboBio (Guangzhou, China) |
| miR-223-3p mimics controls |
|
Ruibo Bio (Guangzhou, China) |
| miR-2909 mimics |
|
Ruibo Bio (Guangzhou, China) |
| miR-2909 mimics controls |
|
Ruibo Bio (Guangzhou, China) |
| miR-223-3p inhibitor |
|
Ruibo Bio (Guangzhou, China) |
| miR-223-3p inhibitor controls |
|
Ruibo Bio (Guangzhou, China) |
| miR-2909 inhibitor |
|
Ruibo Bio (Guangzhou, China) |
| miR-2909 inhibitor controls |
|
Ruibo Bio (Guangzhou, China) |
| STAT3 siRNA |
|
Ruibo Bio (Guangzhou, China) |
| si-IL-6 |
|
Ruibo Bio (Guangzhou, China) |
| si-TNF-α |
|
Ruibo Bio (Guangzhou, China) |
| control siRNA |
|
Ruibo Bio (Guangzhou, China) |
| Lipofectamine 2000 |
11668019 |
Thermo Fisher |
