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. 2019 Feb 13;15(2):e1007598. doi: 10.1371/journal.ppat.1007598

Fig 1. Effects of loss of NHR-8 function on susceptibility of Caenorhabditis elegans to IVM.

Fig 1

(A) Dose response curves to IVM in a larval development assay of nhr-8(ok186) in comparison to the wild-type Bristol N2. Values represent the percentage of L1 reaching the young adult stage after 55 hours of incubation at 21°C within the presence of increasing doses of IVM. Data are mean ± SEM from 6 independent experiments. (B) Microfluidic Electropharyngeograms (EPGs) recorded from wild-type N2 Bristol and nhr-8(ok186) with or without IVM exposure. (C) Analysis of pharyngeal pumping activity (pump frequency) showing hypersensitivity of nhr-8(ok186) mutant to IVM and the rescue of IVM sensitivity by C. elegans nhr-8 cDNA. Pump frequency was compared in worms exposed to control or 0.1 μM IVM using the Nemametrix ScreenChip system. EPG recordings (2–4 min per worm) were started 20 min after the onset of IVM exposure. Data are reported as the mean ± SEM; n = 30–90 worms/group. a p<0.001 vs untreated worms; b p<0.001 vs wild-type Bristol N2.