Table 2. Effect of nhr-8 silencing on susceptibilities of wild-type Bristol N2, IVM-selected (IVR10) and MOX-selected C. elegans strain to IVM.
| Bristol N2 | IVM-selected strain (IVR10) | MOX-selected strain | |||
|---|---|---|---|---|---|
| EC50 IVM (nM) | EC50 IVM (nM) | RF | EC50 IVM (nM) | RF | |
| Control RNAi | 1.5 ± 0.3 | 12.7 ± 1.2 | 8.7 | 13.1 ± 0.4 | 8.9 |
| nhr-8 RNAi | 1.0 ± 0.2 * | 7.1 ± 2.1 ** | 4.8 | 8.8 ± 1.6 * | 6.0 |
| R | 1.3 | 1.8 | 1.5 | ||
EC50 (effective concentration for 50% inhibition) calculated from LDA.
RF (Resistance factor) is the fold resistance relative to the wild-type Bristol N2 strain.
R is the ratio of the EC50 of IVM with control RNAi to the EC50 of IVM with nhr-8 RNAi.
** p<0.01;
* p<0.05 vs Control RNAi. Data are mean ± S.D. from 4–6 independent experiments.