Figure 2. Expression patterns of DIPs and dprs in Drosophila T1 adult leg neuro-musculature.
(A–B) dpr (A) and DIP (B) expression patterns in the Drosophila T1 adult leg for a subset of heterophilic binding partners identified by DIP-Dpr ‘interactome’ studies (Carrillo et al., 2015; Özkan et al., 2013): DIP-α (green) and dpr10 (black); DIP-β (red) and dpr8 (black); DIP-γ (magenta) and dpr11 (black); DIP-ζ (cyan) and dpr13 (black). These DIPs were selected because they are MN-specific in the legs. The expression patterns in this and other panels were generated with MiMIC Gal4 insertions (see Supplementary file 1). (C) Expression of four additional DIPs (DIP-δ, DIP-ε, DIP-η, and DIP-θ) in the T1 adult leg (yellow). In addition to MNs, these DIPs are expressed in leg sensory neurons (DIP-δ, DIP-η, and DIP-θ) or muscles (DIP-ε). (D) DIP-δ, DIP-ε, DIP-η, and DIP-θ expression restricted to glutamatergic MNs neurons in the T1 adult leg using a genetic intersectional approach (see Materials and methods). (scale bar: 50 μm). (E) Heat-map summary of DIP-dpr expression patterns in the T1 leg. Each column represents a distinct DIP or dpr expression pattern and each row represents a specific component of the adult leg-neuro-musculature. MN expression is categorized according to their terminal branching in different segments of the leg: Co, Coxa; Tr, Trochanter; Pr Fe, Proximal Femur; Di Fe, Distal Femur; Pr Ti, Proximal Tibia; Di Ti, Distal Tibia. SN expression is categorized according to their expression in sub-types of SNs (Tuthill and Wilson, 2016): Fe Ch, Femur Chordotonal Organ; Br, Bristle SNs; Ta Br, Tarsal Bristle SNs (campaniform sensilla and hairplate SNs were not included in the expression analysis). Muscle expression is not categorized because two of the three lines were broadly expressed in most muscles. (*) dpr1 is expressed in a single muscle fiber entering the Coxa leg segment.
Figure 2—figure supplement 1. Expression patterns of additional dprs in the Drosophila T1 adult leg.
