Video 1. Live imaging of developing LinA/15 leg MNs between ~25 - 37 hr APF.

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DOI: 10.7554/eLife.42692.004

WT Time lapse in vivo live imaging of developing T1 LinA/15 leg MNs expressing myr::GFP (yellow) between ~25 hr APF (00:00) to ~37 hr APF (12:20) (10 min interval, five fps). In the first frame the Ti-ltm targeting secondary axon bundle is labeled within the white-dotted box demarcating the entire Ti segment. Leg extension is initiated at ~30 hr APF and axons within the secondary bundle begin to defasciculate while filopodial branches maintain physical contact with their muscle targets (Figure 1B–D, Figure 1—figure supplement 1C–E). (scale bar: 50 μm).