Video 2. Live imaging of developing LinA/15 Ti-ltm targeting leg MNs between ~40 - 50 hr APF.

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DOI: 10.7554/eLife.42692.005

WT Time lapse in vivo live imaging of developing T1 LinA/15 Ti-ltm targeting leg MNs expressing myr::GFP (yellow) between ~40 hr APF (00:00) to ~50 hr APF (12:20) (10 min interval, five fps). In the first frame the Ti-ltm targeting secondary axon bundle is labeled within the white-dotted box. The generation of stable terminal branches occurs between ~43 to 45 hr APF, at which point leg muscles are being reorganized into distinct muscle fibers (Figure 1B–D, Figure 1—figure supplement 1C–E). (scale bar: 50 μm).