Video 3. Comparison of WT and DIP-α mutant Ti-ltm targeting leg MNs using live-imaging.
Time lapse in vivo live imaging of Ti-ltm targeting leg MNs, including αTi-ltm, in a DIP-α mutant animal between ~30 hr APF (00:00) to ~38 hr APF (08:30) (10 min interval, five fps). Leg MNs are labeled using VGlut-QF >10XQUAS-6xmCherry (magenta) and αTi-ltm is labeled using DIP-α-T2A-Gal4 > 20XUAS-6XGFP (green) (left: αTi-ltm; right: Merge). In the first frame, both, the Ti-ltm and Ti-tadm, lm (levator muscles), and rm (reductor muscles) targeting secondary axon bundles (magenta), as well as individual αTi-ltm and αTi-tadm axons (green) within these bundles are visible. DIP-α mutant αTi-ltm axons generate dynamic filopodia during leg extension, but show a gradual decline in branching between 30 to 45 hr APF (Figure 6A, Figure 6—figure supplement 1A–B). (scale bar: 50 μm).