Video 4. Live imaging of WT αTi-ltm leg MN between ~35 hr APF to ~45 hr APF.

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DOI: 10.7554/eLife.42692.019

Time lapse in vivo live imaging of αTi-ltm leg MNs in control (Video 4) and DIP-α mutant animal (Video 5), using DIP-α-T2A-Gal4 > UAS-DIP-α and DIP-α-T2A-Gal4/DIP-α^– respectively, between ~35 hr APF (00:00) to ~45 hr APF (Control: 11:30; Mutant: 11:40) (10 min interval, five fps). α-leg MNs are labeled using DIP-α-T2A-Gal4 > 20XUAS-6XGFP (yellow). αTi-ltm and αTi-tadm axons are labeled in the first frame. The DIP-α mutant αTi-ltm axon fails to generate stable terminal branches, while the control αTi-ltm axon begins to generate a collateral branch at ~38 hr APF which stabilizes and extends in length by ~45 hr APF (Figure 6B). (scale bar: 50 μm).