Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
OK371-Gal4 | BDSC #26160 | RRID:BDSC_26160 | |
| Genetic reagent (D. melanogaster) |
Vglut-T2A-QF2 | BDSC #60315 | RRID:BDSC_60315 | |
| Genetic reagent (D. melanogaster) |
10 C12-Gal4 | BDSC #47841 | RRID:BDSC_47841 | |
| Genetic reagent (D. melanogaster) |
dpn > KDRT > Cre;
Act > LoxP > LexA, LexA-myr::GFP; UAS-KD |
PMID:24561995 | ||
| Genetic reagent (D. melanogaster) |
Mef2-QF2 | BDSC #66469 | RRID:BDSC_66469 | |
| Genetic reagent (D. melanogaster) |
13XLexAop2-6XGFP | BDSC #52265 | RRID:BDSC_52265 | |
| Genetic reagent (D. melanogaster) |
10XQUAS-6XGFP | BDSC #52264; this paper |
RRID:BDSC_52264 | VK0027 insertion generated for this paper. |
| Genetic reagent (D. melanogaster) |
10XQUAS-6XmCherry | BDSC #52269; BDSC #52270 |
RRID:BDSC_52269; RRID:BDSC_52270 |
|
| Genetic reagent (D. melanogaster) |
20XUAS-6X-GFP | BDSC #52261; BDSC #52262; This paper |
RRID:BDSC_52261; RRID:BDSC_52262 |
86Fa insertion generated for this paper. |
| Genetic reagent (D. melanogaster) |
20XUAS-6X-mCherry | BDSC #52268 | RRID:BDSC_52268 | |
| Genetic reagent (D. melanogaster) |
MiMIC-T2A-Gal4 lines | BDSC #7838; BDSC #76200; This paper |
RRID:BDSC_78385; RRID:BDSC_76200 |
Additional lines are listed in Supplementary file 1. generated by S.Nagarkar Jaiswal, H.Bellen and M.Courgeon, C. Desplan. |
| Genetic reagent (D. melanogaster) |
DIP-α-A8-Gal4 | This paper | attp2 and 86Fa insertions generated for this paper; See Materials and methods |
|
| Genetic reagent (D. melanogaster) |
DIP-α-T2A-QF2 | This paper | MiMIC Trojan Swaps generated for this paper; See Materials and methods |
|
| Genetic reagent (D. melanogaster) |
DIP-β-T2A-QF2 | This paper | MiMIC Trojan Swaps generated for this paper; See Materials and methods |
|
| Genetic reagent (D. melanogaster) |
DIP-α1-7 also referred to as DIP-αnull2 | PMID: 30467079 | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
DIP-β1-95 | This paper | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
DIP-γ1-67 | PMID: 30467079 | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
UAS-DIP-α-V5 | PMID: 30467079 | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
UAS-DIP-β | VK0027 insertion generated for this paper. |
||
| Genetic reagent (D. melanogaster) |
dpr6,10 - also referred to as dpr6-10L | PMID: 30467079 | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
dpr101-29 also referred to as dpr10null | PMID: 30467079 | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
dpr61-116 also referred to as dpr6null | PMID: 30467079 | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
UAS-dpr10-V5 | PMID: 30467079 | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
UAS-dpr6-V5 | PMID: 30467079 | Generated by the Zipursky Lab | |
| Genetic reagent (D. melanogaster) |
UAS-dpr10 RNAi VDRC | VDRC# 103511 | ||
| Genetic reagent (D. melanogaster) |
tub > FRT .Gal80> | BDSC #38879; BDSC #38880 |
RRID:BDSC_38879; RRID:BDSC_38880 |
|
| Genetic reagent (D. melanogaster) |
QUAS-DSCP-Flp0.2G (attp2) | BDSC #30008 | RRID:BDSC_30008 | |
| Genetic reagent (D. melanogaster) |
tub-Gal80ts | BDSC #7108 | RRID:BDSC_7108 | |
| Genetic reagent (D. melanogaster) |
DIP-α-GFSTF | BDSC #60523 | RRID:BDSC_60523 | |
| Genetic reagent (D. melanogaster) |
dpr10-GFSTF | BDSC #59807 | RRID:BDSC_59807 | |
| Genetic reagent (D. melanogaster) |
R13C09-Gal4 | BDSC #48555 | RRID:BDSC_48555 | |
| Genetic reagent (D. melanogaster) |
hkb-Gal4 | BDSC #62578 | RRID:BDSC_62578 | |
| Genetic reagent (D. melanogaster) |
DIP-ζ RNAi – TriP.HMS01671 | BDSC #38227 | RRID:BDSC_38227 | |
| Genetic reagent (D. melanogaster) |
FRT42D | BDSC #1802 | RRID:BDSC_1802 | |
| Genetic reagent (D. melanogaster) |
FRT42D tubG80, tubQS | PMID: 29395908 | ||
| Genetic reagent (D. melanogaster) |
y,w,hs-Flp1.22, hs-Flp122.2(Chr 2) | Other | Gift from Gary Struhl | |
| Genetic reagent (D. melanogaster) |
Mef2-Gal4 (Chr 2) | Other | Bellen Lab, provided by R.Carrillo |
|
| Antibody | Rabbit polyclonal Anti-Mef2 |
PMID: 7839146 | RRID:AB_2568604 | Generated by B.Paterson; (1:500) |
| Antibody | Sheep polyclonal Anti-GFP |
Bio-Rad | Cat# 4745–1051 | (1:500) |
| Antibody | Chicken polyclonal Anti-GFP |
Abcam | Cat# ab101863; RRID:AB_10710875 |
(1:1000) |
| Antibody | Mouse monoclonal Anti-DIP-α |
PMID: 30467079 | Generated by the Zipursky Lab; (1:20) |
|
| Antibody | Mouse monoclonal Anti-Dpr10 |
PMID: 30467079 | Generated by the Zipursky Lab; (1:500) |
|
| Recombinant DNA reagent |
T2A-QF2-Hsp70 | PMID:25732830 | RRID:Addgene_62944; RRID:Addgene_62945 |
|
| Recombinant DNA reagent |
pJFRC28-10XUAS-IVS-GFP-p10 | PMID:22493255 | RRID:Addgene_36431 |