Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Cell line (H. sapiens)	EPC2	https://www.med.upenn.edu/molecular/documents/EPCcellprotocol032008.pdf		Prof. Hiroshi Nakagawa (University of Pennsylvania)
Cell line (H. sapiens)	CP-A	CP-A (KR-42421) (ATCC )	ATCC:CRL-4027; RRID:CVCL_C451
Cell line (H. sapiens)	OE33	ECACC	ECACC:96070808; RRID: CVCL_0471
Cell line (H. sapiens)	AGS	AGS (ATCC CRL-1739)	ATCC:CRL-1739; RRID:CVCL_0139
Chemical compound, drug	KSFM	Gibco/Thermo Fisher	Cat#:17005042
Chemical compound, drug	RPMI 1640 medium	Gibco/Thermo Fisher	Cat#:21875–034
Chemical compound, drug	Human recombinant EGF	Gibco/Thermo Fisher	Cat#:PHG0313
Chemical compound, drug	Celltracker Orange (CMRA)	Life Technologies/ Thermo Fisher	Cat#:C34551
Chemical compound, drug	Celltracker Green (CMFDA)	Life Technologies/ Thermo Fisher	Cat#:C7025
Chemical compound, drug	Celltracker DeepRed	Life Technologies/ Thermo Fisher	Cat#:C34565
Chemical compound, drug	Image-iTTM FX Signal Enhancer	Thermo Fisher	Cat#:I36933
Chemical compound, drug	Antibody diluent, background reducing	Agilent Dako	Cat#:S3022
Chemical compound, drug	Antibody dilutent	Agilent Dako	Cat#:S0809
Chemical compound, drug	Fluoromount-G	SouthernBiotech	Cat#:0100–01
Chemical compound, drug	DAPI (1 mg/ml)	Thermo Fisher	Cat#:62248	1:1000 dilution
Antibody	Goat polyclonal anti-mouse Alexa Fluor 488	Thermo Fisher	Cat#:A-11001; RRID:AB_2534069	1:400 dilution
Antibody	Phalloidin Alexa Fluor 647	Thermo Fisher	Cat#:A22287; RRID:AB_2620155	1:400 dilution
Antibody	Mouse monoclonal anti E-cadherin	Becton, Dickinson U.K Ltd.	Cat#:610181; RRID:AB_397580	1:400 dilution
Other	25 culture-inserts 2-Well for self-insertion	Ibidi	Cat#:80209
Software	Fiji ImageJ	https://imagej.net/Fiji	RRID:SCR_002285	TrackMate Plugin
Software /Algorithm	Motion Sensing Superpixels	This paper	RRID:SCR_016839	https://github.com/fyz11/MOSES