Figure 1. Treg^Δ142 mouse: validation data.

(A) ChIP-Seq binding profiles (reads/million, input subtracted) for FOXP3 and H3K4me3 and mRNA-Seq (reads/million) around miR142 in Tregs. Genes and super-enhancers are shown below and a scale bar above. Tregs are defined as CD4⁺CD25⁺FOXP3⁺. (B) Flow cytometric gating on YFP (sorted and fixed live CD4⁺ cells) and concomitant/subsequent FOXP3 staining. (C) miR-142-5p expression in naive CD4⁺ T cells and YFP⁺ Tregs in WT and Treg^Δ142 by RT-qPCR. n ≥ 3. *P < 0.05; **P < 0.001, 2-tailed Student’s t test. (D) Total CD3 counts in spleen (n = 4 per group) and peripheral lymph node. n = 7 per group. Nonsignificant, 2-tailed Student’s t test. (E) Total CD4 counts in spleen and peripheral lymph node in absence of disease (<10 weeks; n = 5; nonsignificant, 2-tailed Student’s t test) and presence of disease (>10 weeks; n = 3; nonsignificant, 2-tailed Student’s t test).