(A) Weight charts demonstrating weight loss from 7 weeks of age in TregΔ142 males and females. ***P < 0.001, 2-tailed Student’s t test. n > 10 per group. WT and TregΔ142 data are also shown in Figure 4A. (B) Survival of TregΔ142 and WT littermate control mice. P < 0.001, 2-tailed Student’s t test. Data combined from 2 independent experiments. n > 10. (C) Scurfy-type phenotype seen in TregΔ142 mice (16-week-old female mouse shown); gross splenomegaly and lymphadenopathy seen in TregΔ142 mice. (D) H&E staining of formalin-fixed, paraffin-embedded sections from ear skin, liver, and lung. Original magnification, ×20 (skin); ×10 (lung, liver). WT and TregΔ142 skin, lung, and liver histology are also shown in Figure 6E.