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. 2019 Feb 11;129(3):1278–1294. doi: 10.1172/JCI97642

Figure 7. Induction of antigen presentation by TAMs enhances the accumulation of specific CD8+ T cells at the local tumor site.

Figure 7

(A) The complex of CHP with 9m epitope–containing LPA (50 μg) was intravenously injected with CpG ODN (50 μg) into CMS5a tumor–bearing BALB/c mice (n = 2 mice per group) on day 7. Naive CD8+ cells isolated from DUC18/CD90.1 mice were infused into the same mice on day 8. The tumors were removed from the mice on day 11, and the frequency of CD90.1+CD8+ T cells in the tumors was determined by flow cytometry. Data from 3 experiments were pooled and represent the mean ± SD. (B and C) CMS5a tumor–bearing BALB/c mice (n = 3–4 per group) were treated as in A, and the tumors were isolated on day 11. The concentration of chemokines and cytokines in the tumors was measured by Bio-Plex assay. Circles in the graph indicate the values of the individual tumors, and bars show the mean. *P < 0.05, by 2-factor factorial ANOVA followed by Tukey-Kramer post hoc analysis. (D) Expression of immune checkpoint molecules on CD90.1+CD8+ T cells infiltrating the tumors isolated on day 11 was analyzed by flow cytometry (n = 3–4 per group). Data represent the mean ± SD. *P < 0.05, by 2-factor factorial ANOVA followed by Tukey-Kramer post hoc analysis. The experiments were repeated at least 2 to 3 times with similar results.