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. 2019 Feb 11;129(3):1193–1210. doi: 10.1172/JCI123267

Figure 2. Ptpn2 haploinsufficiency aggravates mannan-induced arthritis in SKG mice.

Figure 2

(A) Clinical score and ankle thickness in male Ptpn2+/+ (n = 13) and Ptpn2+/– (n = 11) SKG mice after mannan injection. (B) Representative images of H&E (top panels) and safranin-O staining (middle panels) of ankles from Ptpn2+/+ (n = 9) and Ptpn2+/– (n = 8) SKG mice with mannan-induced arthritis. Arrowheads indicate synovial inflammation (S), bone erosion (E), and cartilage depletion (C), which are quantified in the lower panel. Scale bars: 500 μm. (C) Micro-CT of arthritic ankles from individual Ptpn2+/+ and Ptpn2+/– male SKG mice with mannan-induced arthritis. Arrows indicate bone erosion or reactive bone deposition that is markedly increased in Ptpn2+/– SKG mice. (D) Quantitative PCR analysis of cytokine gene expression in ankles of Ptpn2+/+ (n = 7) and Ptpn2+/– (n = 8) male SKG mice 35 days after mannan injection. RQ, relative quantification. Compiled data from at least 2 independent experiments are shown in A–D. Arthritis severity was quantified using the area under the curve. Each symbol in B and D represents an individual mouse. Bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by Mann-Whitney (A and B) or unpaired t test (D).