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. 2019 Feb 11;129(3):1180–1192. doi: 10.1172/JCI122694

Figure 2. The +9.5 enhancer WGATAR motif is insufficient for embryogenesis.

Figure 2

(A) Sequences of WT (+9.5+/+) and E-box Ets–mutant [+9.5(E-box Ets)–/–] mice. (B) Sequence of mutant mice. (C) PCR genotyping. (D) Quantification of E13.5 liver cells from +9.5(E-box Ets+/+) (n = 11), +9.5(E-box Ets)+/– (n = 30), and +9.5(E-box Ets)–/– (n = 6) mice. Data are from 5 experiments. (E) E13.5 fetal livers from littermate mice. (F) E13.5 littermates with anemia, hemorrhage, and edema. (G) Gata2 mRNA quantification of fetal liver cells from +9.5(E-box Ets)+/+ (n = 5), +9.5(E-box Ets)+/– (n = 9), and +9.5(E-box Ets)–/– (n = 4) mice. Data are from 3 experiments. Quantitative data are represented as box-and-whisker plots, with bounds from the 25th to 75th percentiles, the median line, and whiskers ranging from minimum to maximum values. *P < 0.05, by 2-tailed, unpaired Student’s t test with Benjamini-Hochberg correction.