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. 2019 Feb 11;129(3):1180–1192. doi: 10.1172/JCI122694

Figure 3. The +9.5 enhancer WGATAR motif is insufficient for HSC genesis and function.

Figure 3

(A) Whole-mount immunostaining of E10.5 dorsal aorta (DA). CD31+ cells are shown in magenta and c-Kit+ cells in green. Scale bars: 100 μm. (B) c-Kit+ cell quantification within the dorsal aorta from +9.5(E-box Ets)+/+ (n = 3), +9.5(E-box Ets)+/– (n = 4), and +9.5(E-box Ets)–/– (n = 4) mice. Data are from 2 experiments. (C) Flow cytometric analysis of E13.5 fetal liver HSCs (Lin−CD41−CD48−Mac1+Sca1+Kit+CD150+) and MPPs (Lin−CD41−CD48−Mac1+Sca1+Kit+CD150–). FSC, forward scatter. (D and E) HSC and MPP quantification (percentage of live fetal liver cells from +9.5(E-box Ets)+/+ (n = 12), +9.5(E-box Ets)+/– (n = 19), and +9.5(E-box Ets)–/– (n = 4) mice. Data are from 3 experiments. Quantitative data are represented as box-and-whisker plots, with bounds from the 25th to 75th percentiles, the median line, and whiskers ranging from minimum to maximum values. *P < 0.05, **P < 0.01, and ***P < 0.001, by 2-tailed, unpaired Student’s t test with Benjamini-Hochberg correction.