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. 2019 Feb 11;129(3):1180–1192. doi: 10.1172/JCI122694

Figure 8. +9.5 enhancer Ets motif–dependent HSPC regeneration.

Figure 8

BM was harvested 9 or 11 days after vehicle (PBS) or 5-FU (250 mg/kg) treatment. (A) Flow cytometric analysis of LSK cells (Lin−CD48−Sca1+Kit+), HSCs (Lin−CD48−Sca1+Kit+CD150+), MPPs (Lin−CD48−Sca1+Kit+CD150–), LS–K cells (Lin−Sca1–Kit+), MEPs (Lin−Sca1–Kit+FcγR–CD34–), CMPs (Lin−Sca1–Kit+FcγR–CD34+), and GMPs (Lin−Sca1–Kit+FcγR+CD34+). (B) LSK, HSC, MPP, LS–K, MEP, CMP, and GMP quantification (percentage of live BM cells). n = 8–10 per genotype and treatment; data are from 5 experiments. Quantitative data are represented as box-and-whisker plots, with bounds from the 25th to 75th percentiles, the median line, and whiskers ranging from minimum to maximum values. *P < 0.05, **P < 0.01, and ***P < 0.001, by Tukey’s multiple comparisons test.