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. 2019 Feb 11;129(3):1129–1151. doi: 10.1172/JCI121987

Figure 11. Inhibition of PtenK27-polyUb alleviates STZ-induced diabetic nephropathy.

Figure 11

(A) Scheme of the experimental approach. Three groups of animals were compared: PtenWT/K80R with vehicle injection, PtenWT/K80R with STZ injection, and PtenK80R/K80R with STZ injection. (B) Measurement of ACR of PtenWT/K80R + vehicle injection, PtenWT/K80R + STZ and PtenK80R/K80R + STZ animals. Error bar indicates SD; n = 8, 9, 8 animals, respectively (1-way ANOVA). (C) Representative images of H&E staining, PAS staining, and immunofluorescence staining using indicated antibodies in PtenWT/K80R + vehicle injection; PtenWT/K80R + STZ and PtenK80R/K80R + STZ kidneys. Scale bars: 50 μm (black) and 100 μm (white). White circle: glomerular; black arrow: expanded mesangial area. (D–H) Statistical analysis of PAS-positive mesangial area (D), staining intensity of α-SMA (E), staining of Ub-PTEN (K80) (F), MEX3C (G), and TWIST (H) per Na+K+-ATPase–negative glomerular (left panels) or –positive tubules (right panels) per visual field. Error bars indicate SD; n = 8 animals; 6 independent fields per animal were calculated (1-way ANOVA). NS indicates P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.