Skip to main content
. 2019 Feb 21;12:1475–1495. doi: 10.2147/OTT.S190847

Figure 5.

Figure 5

NE contributed to SACC cell proliferation and migration via β2-AR.

Notes: (A, B) MTT assay showed that incubating SACC-83 and SACC-LM cells with different concentrations of NE (0.1–100 μM) for 48 hours promoted SACC-83 and SACC-LM cell proliferation in a concentration-dependent manner. Treatment with 10 μM NE significantly promoted the proliferation of SACC-83 and SACC-LM cells. ICI118,551 inhibited the proliferation of SACC-83 and SACC-LM cells in a concentration-dependent manner. Treatment with 10 μM ICI118,551 significantly inhibited the NE-induced proliferation of SACC-83 and SACC-LM cells. (C) Transwell migration assay showed that NE promoted SACC-83 and SACC-LM cell migration in a concentration-dependent manner. Treatment with 10 μM NE significantly promoted SACC-83 and SACC-LM cell migration. The number of migrated cells was quantified. Original magnification, 200×; scale bar =50 μm. (D, E) Wound-healing assay comparing the migration abilities of SACC-83 and SACC-LM cells treated with 10 μM NE and/or 10 μM ICI118,551. The wound-closure percentage was quantified at 36 hours after scratching relative to that at 0 hour. Original magnification, 40×; scale bar =200 μm. *P<0.05, **P<0.01, ***P<0.001. The error bars indicate the SD.

Abbreviations: β2-AR, β2-adrenergic receptor; NE, norepinephrine; SACC, salivary adenoid cystic carcinoma.