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. 2019 Feb 21;12:1475–1495. doi: 10.2147/OTT.S190847

Figure 6.

Figure 6

NE contributed to PNI in SACC via β2-AR.

Notes: (A) Transwell PNI assay comparing the PNI abilities of SACC-83 and SACC-LM cells treated with 10 μM NE and/or 10 μM ICI118,551, with quantification of the number of invaded cells at 24 hours. Original magnification, 200×; scale bar =50 μm. (B, C) DRG coculture models comparing the PNI abilities of SACC-83 and SACC-LM cells treated with 10 μM NE and/or 10 μM ICI118,551. The red lines indicate the edges of the SACC colonies, the green lines indicate the edges of outgrowing DRG neurites, and the black lines indicate the total distance between the DRGs and the cancer colonies. The nerve invasion index (α/γ) and DRG outgrowth index (β/γ) were calculated as described in the Materials and methods section. (D, E) The nerve invasion index and the DRG outgrowth index were quantified at 72 hours relative to that at 24 hours. Original magnification, 40×; scale bar =200 μm. *P<0.05, **P<0.01, ***P<0.001. The error bars indicate the SD.

Abbreviations: β2-AR, β2-adrenergic receptor; DRG, dorsal root ganglia; NE, norepinephrine; PNI, perineural invasion; SACC, salivary adenoid cystic carcinoma.