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. 2019 Feb 21;12:1475–1495. doi: 10.2147/OTT.S190847

Figure 9.

Figure 9

β2-AR mediated NE-induced EMT and upregulation of MMPs in SACC cells.

Notes: (A, B) qRT-PCR analysis of expression of the indicated genes in SACC-83 and SACC-LM cells after transfecting with β2-AR siRNA for 24 hours. GAPDH expression was detected as an internal control. The mRNA expression levels of E-cadherin, N-cadherin, Vimentin, Slug, MMP2, and MMP9 were quantified and expressed as fold-changes compared with control groups. (C, D) Western blot analysis of the expression of the indicated proteins in SACC-83 and SACC-LM cells after transfecting with β2-AR siRNA for 48 hours. GAPDH expression was detected as an internal control. (E, F) Protein expression levels in SACC-83 and SACC-LM cells were quantified and expressed as fold-changes compared with control groups. *P<0.05, **P<0.01, ***P<0.001. The error bars indicate the SD.

Abbreviations: β2-AR, β2-adrenergic receptor; EMT, epithelial–mesenchymal transition; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MMP, matrix metallo-proteinase; NE, norepinephrine; qRT-PCR, quantitative real-time polymerase chain reaction; SACC, salivary adenoid cystic carcinoma.