Figure 3.

Transepithelial penetration of indomethacin in IMC-NPs at 4°C and 37°C using HCE-T cell monolayers.

Notes: (A) Changes of TER in HCE-T cell monolayers treated with IMC-NPs 4°C and 37°C. Accumulation (B) and penetration (C) of indomethacin in HCE-T cells treated with IMC-solution, IMC-MPs and IMC-NPs, at 4°C and 37°C. Number (D) and size frequencies (E) of indomethacin nanoparticles in the basolateral side at 4°C conditions. n=6. *P<0.05 vs 37°C conditions for each category. ^#P<0.05 vs IMC solution at 37°C conditions. The accumulation of indomethacin tended to be less at 4°C, and transepithelial penetration was significantly prevented at 4°C.

Abbreviations: IMC-MPs, ophthalmic formulation containing indomethacin microparticles; IMC-NPs, ophthalmic formulation containing indomethacin nanoparticles; con, concentration; TER, transepithelial electrical resistance.