Figure 5:

RNA from human MDM isolated from previously frozen PBMC was collected from 12 healthy, and 12 cART-treated donors to quantify expression of DRD1, DRD2, DRD3, DRD4, and DRD5. Expression level of each receptor is normalized to the level of the endogenous control 18s (ΔC_T), and data is represented as the transformed value 2^−ΔC_T. (A, B) Healthy and cART donor MDMs isolated from previously frozen PBMC express significantly greater levels of DRD5 than any other receptor. Significance was determined via Kruskal-Wallis test followed by Dunn’s multiple comparisons test, (*p<0.05, **p<0.01, p<.001 ***, p<0001****). (C) In MDM isolated from previously frozen PBMC, cART donors express significantly greater DRD5 than healthy donors. Significance was determined via individual unpaired Mann-Whitney tests between healthy and cART groups for each dopamine-receptor subtype. There was no significant difference in expression for any other dopamine-receptor subtype (*p<0.05). (D) Across all healthy donors, MDM isolated from previously frozen PBMC express less DRD1 and DRD2 than freshly isolated MDM. Significance was determined via individual unpaired Mann-Whitney tests between fresh and frozen groups for each dopamine-receptor subtype. There was no significant difference in expression for other dopamine-receptor subtype (*p<0.05).