Table 5.
Cytokine levels and effect of dopamine on cytokine production in MDM isolated from healthy and cART-treated donors
| Cytokine | Effect | Concentration in UT cellsa | Concentration in LPS- stimulated cellsa |
Fold change in response to dopamineb | Simple main effect of dopamine treatment?c |
Interaction between donor group and treatment?c |
Simple main effect of donor group?b |
||
|---|---|---|---|---|---|---|---|---|---|
| IL-6 Health cART |
Inflammatory | 34,260 (+/− 11,560) 20,095 (+/− 10,579) |
689,549 (+/− 306,212) 422,130 (+/− 280,243) |
10−6 2.6x +/− .71 (.1066) 5.59x +/− 3.53 (.094) |
10−7 2.7x +/− .85 (.0512) 1.4x +/− .41 (>.999) |
10−8 2.1x +/− .60 (.424) . .87x +/− .16 (.735) |
Yes, F(4,76)=3.27, p=.0157* |
No, F(4,76)=1.75, p=.1474 |
Yes, F(1,19)=8.6, p=.0085** |
| IL-1β Health cART |
Inflammatory | 1,396 (+/− 198)d 741.8 (+/− 161)d |
34,030(+/− 11.153)d 17,352 (+/− 3,848)d |
1.8x +/− .72 (.865) 2.6x +/− 1.1 (.184) |
2.6x +/− 1.4 (.794) 1.5x +/− .38 (.774) |
1.4x +/− .41 (.998) 1.3x +/− .195 (.913) |
No, F(4,80)=1.2, p=.3187 |
No, F(4,80)=.30, p=.8739 |
Trending, F(1,20)=3.87, p=.0631 |
| IL-10 Health cART |
Anti-inflammatory | 1,256 (+/− 322) 1,107(+/− 251) |
13,620 (+/− 3,235) 26,789 (+/− 12,131) |
.98 +/− .063 (.929) .85x +/− .084 (.104) |
1.1x +/− .12 (.998) 1x +/− .08 (.999) |
No, F(2,38)=1.64, p=.2065 |
No, F(2,38)=.89, p=.4207 |
No, F(1,19)=.04, p=.8347 |
|
| CXCL-8 Health cART |
Neutrophil chemoattractant |
907,692 (+/− 174,774) 1,086,978(+/− 381,335) |
2,932,511 (+/− 599,710) 5,427,559 (+/− 1,895,066) |
1.7x +/− .34 (.610) 2.7 +/–1.2(.908) |
1.5x +/− .27 (.851) 1.7x +/−.5 (.992) |
.96x +/− .14 (.886) .64x +/−.12 (.047)* |
Yes, F(4,84)=3.77, p=.0072** |
No, F(4,84)=.75, p=.5618 |
No, F(1,21)=1.28, p=.2708 |
| CXCL-10 Health cART |
Monocyte chemoattractant |
92,338 (+/− 43,565) 81,249 (+/− 47,756) |
537,267 (+/− 295,600) 216,439 (+/− 131,774) |
1.6x +/– 2.4 (.061) 1.4x +/− .29 (.807) |
1.8x +/− .35 (.054) 1.3x +/− .21 (.893) |
1.3x +/− .16 (.577) .86x +/− .13 (.334) |
Yes, F(4,84)=2.91, p=.0262* |
No, F(4,84)=.98, p=.4204 |
No, F(1,21)=.50, p=.4874 |
a
Mean concentration in pg/mL per 106 cell supernatant (+/−SEM)
b
Fold change relative to untreated +/−SEM (p-value, Dunnett’s multiple-comparison test)
c
F(DFn,DFd), p-value
d
Mean concentration in pg/mL/μg of protein per 106 cell lysate (+/−SEM)