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Sample Volumes and Concentrations of PAZ and RNA needed for ESMA.
| Protein concentration (nM) | PAZ protein added (μL) | 1x binding buffer added (μL) | RNA(s) of interest added (μL) |
|---|---|---|---|
| 500 | 1.0 (10 μM stock) | 9.0 | 10.0 (500 nM stock) |
| 600 | 1.2 | 8.8 | 10.0 |
| 700 | 1.4 | 8.6 | 10.0 |
| 900 | 1.6 | 8.2 | 10.0 |
| 1250 | 2.5 | 7.5 | 10.0 |
| 2000 | 4.0 | 6.0 | 10.0 |
| 2500 | 5.0 | 5.0 | 10.0 |
| 3000 | 6.0 | 4.0 | 10.0 |
| 6000 | 3.0 (40 μM stock) | 7.0 | 10.0 |
